so the ones that had the highest ec the highest salt growth had the strongest the darkest colors
richest aromas the strongest flavors have you ever done a testing on terpene profiles
after harvesting in a slightly slower with a slow cure like an actual slow cure with all the foliage
on the the plant back in the day they were showing that the main reason that they were the females
were producing the trichomes were to attract the male if there was a male in the vicinity they
would try to produce terpenes to let them know that they were nearby i was working with a big
indoor grower and they were having problems with one room one of their flower rooms was
underperforming it just would stall out then come back there were some symptoms of deficiency let
me come in as a consultant to try to help them so i did some leaf analysis distress actually affect
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and much much more i did one experiment years ago too knocked out of fire but it
was with cannabis specifically where someone had me do the experiment for them and we tried tried
three different ecs it have strength nutrient full strength nutrient and extra strength nutrient
in the side-by-side same environment and the one that had the highest ec created the most
stress on the plan since some fault stress at the root zone it had the darkest colors
the richest aromas the strongest flavors the most terpenes and it wasn't like oh i think i see a
little difference here there was a big obvious difference so back then the early days you repeat
that for me one more time i'm sorry you you said i thought that was really interesting i thought
whenever someone said something interesting i want to repeat it yeah i'll repeat it we did a i did an
experiment with ec you know low half strength but it says on the bottle full strength what it says
on the bottle and extra strength it says on the bottle we grew them side by side two different you
know the same strain everything was the same same cultivar but uh to see what how it affected down
at the very end the one that had the highest salts that creates a salt stress and that salt stress
makes it harder for the plant to take up water and when the plants under stress it makes antioxidants
to protect itself from stress and some of those antioxidants are the anthocyanins the purple
pigments uh some of them are flavonoids some of them are even the terpenoids
so the ones that had the highest dc the highest salt growth had the strongest the darkest colors
richest aromas the strongest flavors what was the yellow like the yield was similar a little bit
less than the medium strength i didn't do hundreds of them i wish i would have had a real control but
it was just when i do a smaller control i cut out whatever the biggest plan is the monster whatever
the runt is and i just average what's in between yeah with that but but the point was the thing was
it wasn't a it wasn't a subtle difference it was a big noticeable difference it was
you can easily see it you can easily smell it it was no question about it so anyway later on
i applied this because back then we were able to there was kind of a gray area we were able to sell
our overage to the dispensaries you know we still had to stay in our limits but we could do that
they can't do that anymore so but what happened was that it was really high prices
at the beginning when that started to happen for the growers oh man i can make some money
just with my ovaries and then though as we went along they started lowering prices because there
was a supply and demand but anyway you go into the dispensary and they say well here's our top shelf
so much per gram here's our bottom shelf so much program where does yours fit in i said oh this is
somewhere in here so i said i can two complete that again i'm going to go with a purple string
because i know what to do right i know exactly what to do how to grow these
so i picked a i think it was granddaddy purple or something like that i push my ecs you know right
to the edge you know they can handle it they could handle that yeah and i changed my light because
i knew too i've been lighting experience the blue the oh the uv b into the blue spectrum stimulates
the plant to make the precursor molecules to the anthocyanins the purple pigments so i changed my
lighting at the end had more blue last couple of weeks or less at least a week and a half
i have my ec high really dark colors and aromas took that into the dispensary in my
jar so i have a sample so do you have any other purple strains oh yeah we have a few
let's see them took it down they looked at mine oh look at the color they open it up
i got the top price every time you created your own category it went one level higher the hardly
it was but i will tell you this this is again i'll tell you a secret here on the whole world a secret
but did just because it had the darkest colors and the strongest terpenes the aromas and flavors
it wasn't the best because it was too much it was like some of those terpenes were so expressed that
they were in your face and covering up some of the more subtle terpene absolutely absolutely
i i've noticed that often when i'm when i'm using and it's like it's an amazing product
but it's so overwhelming the flavors in my mouth that i genuinely can only i can't use it as much
as i as i would use another product and and oh and i can't and i i'm someone that likes to use
the same product for like an ounce or two you know i like to stay consistent through my entire
month two-month experience and then make a subtle switch and the that product was just
um yeah i couldn't use it i could only you know use up seven grams eight grams before i had to
move on to another strand because the flavor is just too overwhelming for me and that's very
interesting you say that yeah so i mean it was but that's what people wanted when they were shopping
they look at the color and they do the sniff sniff test and they go oh yeah it's most expensive than
i want for this one but they didn't realize one that maybe wasn't quite as dark or strong
they actually might have a better profile of terpenes and be more and you know if you're really
paying attention you can you'll enjoy it more so you know again mark it once sort of but
i you know so it sounds like it's uh anthocyanin production can be triggered by different stresses
do you think that the different stresses of the different triggers would help produce
different types of uh sweetnesses or terpenes or whatever uh flavonoids and all that because
this is my mine i just noticed this this past week uh i have a mini split cooler and i had
a dry setting that i accidentally left it on overnight and i brought the room down to like 55
and then after that they did they weren't drippy or anything after that they were still
tablets was so good but they started producing anthocyanins like really heavy and then as i was
deleting yesterday i was getting purple fingers so uh whoa yeah that was the first time that i've
seen that so i was like i was de-leaping and i was like whoa there's my fingers are purple
like like i stuck them in jelly or something so i'm wondering if the different triggers have
caused different types of uh outcomes i guess nothing it's mainly a stress response
so you could use drought stress you could use heat stress you could use salt stress you know
they all have that effect the way you were doing though by cooling them cooling it down
it was degraded the plant started to grade some of the chlorophyll just like in the fall colors
i'm sure it is easy to see as well too built colors because it is a combination of easy as
well high ec because i do do uh i do do this organic so i'll start out with vegetating with
uh organics and then i'll start to add in like quarter strength of like bottle nutrients if i
need them and then do that but i do like like what you're saying with the highest salt stress and
it's i see that i see it producing a lot better but you see that's kind of the opposite of the
flushing concept yeah have you ever done a testing on uh terpene profiles after harvesting in a
slow re with a slow cure like an actual slow cure with all the foliage on the the plant like instead
of stressing the plant out before you harvest it max out the yield then stress it out through
the curing through the soil curing process that's always i'm a big proponent on this and that's why
i encourage the whole channel have you actually done experiments around this that can you know
further that thought process around uh terpene profiles uh yeah that that's something that i'm
is constantly in my from my mind that's some of the experiments i want to do now
i really want to improve terpene production and the i really fresher cooler fresh and cool you
can have preserve the terpenes they're not going to burn off as much maybe experimenting with
nighttime temperature cooling it down a little bit more toward the end um but i i think it's
really you know there's some things you can do to accelerate the terpene production but we need to
preserve the terpenes that are there and that's with the curing that's with the drying and curing
i tell people that that it creates a cocoon experience that protects the flower and keeps the
volatile compounds on the plant and then through with all the microbiology on the plant and through
recent discoveries that's where my thought process and the water in the stem water in the leaves the
plant continues to keep growing and and will and that my thought process it produces that chemical
that makes the plants get irritated and then at that point it then opens up even further further
access to more terpenes because we've maxed out the health the yield what it could produce on its
own through that and then like you said then through the stress if if you ever do that and
really critically go through it through testing i would love to participate in that test or just be
you know be aware of if you do it in the future yeah i want to i'm going to be doing
those are the next experiments i'll be doing because my next priority is terpenes because
i think that's going to be the there's been so much emphasis on thc cannabinoids cannabinoids
i'm i'm really i'm really big into i produce rosin and all that so i'm really big into the
the science of like what terpenes are and everything they do they use the pheromones of
the terpenes to communicate to the other plants and uh back in the day they were
showing that the main reason that they were the females were producing the trichomes were to
attract the male if there was a male in the vicinity they would try to produce the terpenes
to let them know that they were nearby and if you have a bunch of females in the area they
will compete with each other so what they did is they had a male in a separated room with a with a
filtered hvac system so the pheromones were still able to get through it's just the pollen
wasn't so what they found is that they were all producing different types of terpenes to try to
to win over that male's pollen because that's just what they they concluded so uh and then there was
studies that were popping up from plants in africa they were being eaten
into extinction by the animals nearby so they started producing a terpene
through the leaves to communicate to each other to start producing to produce a neurotoxin so it
started killing the animals that were eating it so this to save itself so they're noticing that
different stresses can cause plants to produce different terpenes through communication processes
yeah that's true they're communicating with other plants they're communing with the microbes
and the microbes are communicating with them we don't even hardly know anything yet it's
crazy but they they are there is there's a response if one of them is being attacked
so it starts uh immune response in that plant but it will also start producing
specific terpenes to warn the other plants in the area that they're under attack so start making uh
secondary metabolites make these lavender yeah uh i will uh one thing what was it um
oh yeah um i was out working out west with with some growers this has to do with terpenes and
cannabinoids both i was working with a big indoor grower one of the biggest ones south southern
oregon and they were having problems with one room one of their flower rooms was under performing
it just would stall out then come back there were some symptoms of deficiency let me come in
as a consultant to try to help them so i did some leaf analysis i took uh some because i had an icp
oes spectrometer in my lab i was i had my own toys so i took the ones that showed some symptoms some
striping on them i took some from the same part of the plant so they were healthy did a side-by-side
test the one that had the symptoms had one-third the manganese of the other one manganese that's a
trace element that said oh that's interesting but why aren't they taking up the manganese
it was during transition and what they were doing they were going with a lot of the blue for veg
to a lot more of the red for flower in the flower room well the blue stimulates the stomata to open
so they're going to transpire and take up some of those hard to take up elements like manganese
and so all of a sudden there's less blue to stimulate the opening they also raise the co2
from the lower levels to high levels so what happens and if they then adapt it that the
stomata tend to close they didn't have the blue to tell them to open so they weren't
taking up the nutrients so what i did is i had them at 30 percent more blue during transition
had to ramp up the co2 instead of going up all at once if some plant could adapt the new environment
and of course we had a manganese deficiency so i had them used a manganese amino acid chelate
to do a foliar spray with it to fix it up quick so the end of the story was they that went from the
mo an underperforming room to the best room ever okay now here's the icing on the cake
at that time they were also in a contest for the whole state of oregon called the grower's class
and all the growers in the contest had the same genetics and they did test you know
you grow it and in the end we're going to do the test for terpenes and everything they won
first place in the state of oregon that year for terpenes and they won first place in the state
for thc wow both wow well wait a minute let me go back and look at this because i always kind
of wanted to do that experiment i went back and i have a metabolic pathway chart over there on that
wall you can't see so went back to see where is that where the terpenes are produced and manganese
is a cofactor it turns on the enzymes to make the those terpenes those pre terpenoids
and 300 coenzyme reactions megane is responsible for in the plant so if an enzyme is turned on
it can do a thousand chemical reactions per second so it's again the efficiency of using it
so because i added the manganese up to the optimal levels for that room that particular
plant was getting closer to what was ideal and because of that when they went first in the state
that's a big contest the whole state of oregon is a pretty good pretty good producer
when i'm looking at water analysis i don't want to see more than 70 parts per million of chloride
that's kind of my my limit and no more than 50 parts per million of sodium