The Zetasizer Nano ZSP is a new, premium addition to the Zetasizer Nano range.
This video demonstrates how the new system pushes the capabilities of size
and zeta potential measurement to new limits for nano particles and biological molecules.
It introduces two new measurement types protein mobility and microrheology.
For colloid, nano particle and macro molecule characterization
our customers tell us that the Zetasizer Nano instruments are the most user-friendly systems available.
The ZSP is no different.
Samples are easily introduced via the top opening lid
and the side opening train provide storage for spare cuvettes or measurement cells.
Cells are available for a range of applications.
A capillary cell is entirely disposable in order to eliminate any possibility of cross-contamination,
a dip style cell uses low-cost disposable cuvettes
and the surface zeta potential cell enables a measurement of material surfaces
A high concentration cell simplifies and minimizes sample preparation.
A range of cuvettes is available to match your application requirements.
Low volume, disposable or chemically resistant quartz.
These can also be used for molecular weight measurements as well as the flow cell
for when the Zetasizer is used with the auto titrator or with Size Exclusion Chromatography (SEC)
Let's just remind ourselves why the Zetasizer range is so successful
by showing how easy it is to make a size measurement.
That's one thing that hasn't changed with the ZSP.
The sample can be measured in a range of cuvette volumes down to 12 microliters.
Operators have the option to run a standard measurement.
However, a dedicated protein standard operating procedure
gives even more information about the sample.
The system will perform some short tests and automatically optimize the measurement conditions
Every run generates a comprehensive report that gives a range of information about a sample
including its hydrodynamic radius of course.
The new Zetasizer Nano ZSP has over ten times the sensitivity of its predecessor
which means it can measure samples that are much more dilute than ever before.
This is particularly important for protein measurements.
A new method of sample introduction, the diffusion barrier technique, has a small sample volume of 20μl
The folded capira cell provides the benefits of the accuracy of measurement of a capillary cell
with the ease of use of a disposable cell.
Being fully disposable, including the electrodes, the folded capillary cell prevents contamination
from components of previous samples and ensures measurement accuracy.
Dedicated protein utilities included with the Zetasizer Nano ZSP as standard
allow the user to extract extra information about the structure of the molecule
and the expert advice system also gives status and guidance on the quality of the data
both during the measurement sequence and after the result is presented.
Microrheology is new measurement option available for the Zetasizer Nano ZSP
Microrheology involves tracking the motion of dispersed trace of particles in a fluid
using dynamic light scattering to extract the rheological properties of the sample.
Dynamic light scattering microrheology has benefits for the determination of rheological properties
of low viscosity, weakly structured, complex fluids, such as, polymers and proteins in solution.
Microrhology measurements can be undertaken on very small sample volumes
and provide very high frequency data which is inaccessible by other techniques.
A microrheology standard operating procedure is provided in the software
which uses both electrophoretic light scattering and dynamic light scattering measurement stages
in order to set up robust methodology for a particular sample time.
The microrheology standard operating procedure is configured with user guidance
to ensure that tracer particles are well dispersed and are not interacting with the sample.
An expert advice system also gives guidance on the quality of the data.
The microrheology measurement step records the correlation function
of tracer particles of known size dispersed in the sample.
which is related to their mean square displacement or MSD.
From the MSD data, the rheological properties of the samples are calculated
using the generalized Stokes-Einstein relation.
Results are obtained for visco elastic moduli G Prime and G double prime
which can highlight material, micro structural changes, such as, protein aggregation in solution
and the complex viscosity (Eta Star) which can highlight non-newtonian flow properties.
The new high sensitivity addition to the Zetasizer Nano range
is coupled with new measurement types to give superior performance.
it's improved optical design enhances performance for protein measurements.
The flexibility and range of the measurement types, together with the exceptional performance
means the Zetasizer Nano ZSP system will handle all of your DLS applications