hey thanks for watching this video goes
over the processing of marks specimen
that was obtained from dr. trainer
during the most procedure if you haven't
already seen dr. trailer do the
operation when he removed the sample go
back and watch that first then come and
watch the processing of the tissue where
we see if you got the whole thing so
what I'm going to do is log the patient
into the logbook and i also find the
patient and accession number and then
just to keep the orientation of the
tissue all map the tissue and how I'm
going to ink it so the doctor knows what
a top and bottom left and right so do
yellow on the left blue on the right
when I inc the tissue its kind of DAB
the extra blood off
and then I'm going to make the cut that
I showed on the map which is at the
bottom so here's the 12 o'clock i'm
gonna keep orientation and that's the
cut that i have on the map and then I'm
just going to relax the tissue tissue
will naturally tense up once it's cut
out of the patient
I'm just going all the way around and
then i'm going to ink the tissue just
like i have on the map so yellow on the
left you on the right
and now we're going to free the tissue
so it's going to make sure that all the
epidermis is down on the side so that
the doctor can see the full margin
when I just check the back to make sure
there's no bubbled and i'm going to face
the tissue at the bottom of the Christ
acts so that I could stick to the spies
that's just freezes
yeah I haven't frozen it yet but i will
eventually i'm just going to freeze the
one that I just put down at the bottom
so just take some of the media put it on
the choke
can do the same thing to the tissue
make some last-minute adjustments
put media on the tissue as well
and then I'm going to combine the two
and three
springwood night yes
and then i'll use this heat sink
put it on top of the tissues and make
sure that it gets as frozen as we can
get it so that will need to free for a
few minutes and then we already have one
that's frozen i'm here at the bottom of
your cooking show its previously
prepared yeah exactly i'm going to mount
it on the micro 2mon
just screw it and marrow
as hard as I can
and then there's a blade here so i
wanted to take the slide off of far away
from the blade as I can
let's put my hand there the palm of my
hand to warm up the slide so it's easier
to take off comes right off
then I cut off the exit media around the
tissue
what does that mean this is Oct matrix
the plastic not too sure
and im Welling the tissue away from the
boys and then i'll just make some
necessary adjustments like tighten the
check to make sure that it's not least
now i need to angle the tissue parallel
to the blade so that we can get the
tissue faced off correctly
you can see the blade is hitting at the
bottom you can adjust it so that it's
hitting the tissue evenly
now I got a full face of the tissue
sprite with the liquid nitrogen to
ensure that it as cold as it can get
so the doctor usually like to see six
cuts exceptions of each tissue making
sure the blade is cold
you know help is the places are yes I'm
cutting out of three which is 6 micron 6
micron yes
take it
those things are actually cutting
through cell not between yeah
so we want to make sure that we're
getting the full face petition including
the fat in the middle so it's like using
the American companies
no trial yeah
it will lay the glass on innocent
yeah i'm just making sure I got all the
epidermis and was all the necessary fat
in a doctor to speed there's any cancer
now I'll take the 6 section cut that I
made
I'll put the slides into the slide
holder
we're going to put in alcohol bath so
that the issue can dry out and removed
all the water from the tissue now we've
got in there for a minute and where you
have a tissue that we were working on
I maybe went through the alcohol the
water the hematoxylin and all the water
bath so now i'm just going to run it
through the the ESN
the same different types of elves
different colors
yes so the purple stain nucleic solved
and the ESN will see more like the
cytoplasm and the ESN is a counter thing
to bahama toxins you describe your parts
of ourselves that the different colors
yeah the way you'll be able to tell the
cancer that has more usually the council
be more purple purplish much prettier
once they get the state will cover
something the sides
can be there ready to be with that how
this process works is to consider a bowl
of soup and what we've removed on the
patient is a bowl shaped piece of tissue
the soup in the middle is the tumor and
the margin around it think of that as
the China of the bowl and what rachel
has done is she's taken that bowl and
flattened it into a plate so we've taken
three dimensions into two and now she's
slicing from the bottom of the plate up
towards the soup when I look under the
microscope of all I see is the China of
the plate the surgical margin i know we
have clear margins in all tissue planes
but if I see any soup leaking through
based upon the structure of the tissue
in the mouth i can know precisely where
to go back on the patient and remove a
little bit more and as
Rachel the technician cuts more and more
she's getting closer and closer to the
super the tumor in the bowl and so far
there's no sign of skin capture all
looks like normal healthy tissue and
this appears to be very good news for
our patient because they've seen no sign
of residual skin cancer meaning on the
first removal we got around and below
his skin cancer
no skin cancer cells and thanks for
watching
now that we know that mark does not have
any cancer let's go tell us that will be
in the next video be sure to click and
watch that what is up
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