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- Hi, I'm Brian.
- And I'm David.
Welcome to the Illumina Solutions Center.
We're here to give you a behind-the-scenes introduction
to the newest innovation
in the world of next-generation sequencin g from Illumina,
the iSeq 100.
The iSeq 100 leverages
some of our greatest technological advances
in our smallest and most accessible system to date.
Building on our trusted SBS chemistry and combining it
with our latest single channel CMOS detection technology,
the iSeq 100 offers researchers
a compact and cost-effective benchtop sequencing solution.
The iSeq 100 is easy to use,
and provides up to four million reads in about 17 hours
for a two by 150 Basepair run.
This supports a wide array of applications
such as viral sequencing, small panels
and small whole genomes.
- That's pretty impressive, David.
What I'm most excited about is the easy install and set up
that allows me to quickly get up and running
with the iSeq 100,
as well as the fact that the platform is so small
it can fit just about anywhere in my lab.
- Show us how easy it is
to set up and begin a sequencing run.
You can highlight some features of the consumables
and instrument itself,
and demonstrate what the workflow might look like
for a typical run.
- For the folks out there
that have seen many of our benchtop sequencers,
a lot of what I'm about to show you might look familiar.
Not only did our development team
combine SBS chemistry and one channel CMOS detection,
but they also optimized the workflow
by utilizing the cartridge-based reagent delivery system
used by the MiniSeq, MiSeq and NextSeq.
This makes the process of setting up a run easier than ever.
We'll start with the cartridge.
The cartridge, which has all of your frozen reagents,
will be shipped on dry ice and stored at minus 20 degrees.
It can be thawed in the refrigerator overnight
for a run to be started the next day.
Or, can be placed in a room temperature water bath,
in its storage bag, just a few hours before the run.
Setting up the prepared libraries is very similar
and just as easy as our other benchtop systems.
The reagent cartridge and flow cell
both contain a unique RFID,
making tracking of consumables more convenient.
There's no longer a need to use a barcode scanner
for the information to be entered into the run setup screen.
We'll now take a look at the sequencer itself.
The first feature you can't help but notice
is that the system only takes
about one square foot of space.
The footprint is quite a bit smaller than a MiniSeq,
allowing you to free up bench space.
Its self-contained design requires no additional wash steps,
and includes everything for your sequencing run.
To set up a run,
the prepared library is pipetted into the library well.
There is no need to denature your library before pipetting
because it's done on board the system,
making setup a breeze.
With the intuitive user interface,
starting a run is super easy.
To start, touch the Sequence icon.
The system will prompt you to load your prepared library
into the thawed iSeq 100 cartridge.
Next, insert your flow cell into the cartridge.
With the keyed design of the flow cell,
there is virtually no chance
that the flow cell and cartridge
can get assembled incorrectly.
Load the cartridge containing your library and flow cell
on the tray and press the Close Door button.
While your cartridge is being initialized,
you can enter in your sequencing parameters
or access a previously configured run.
Now you're ready to hit the Start Run button
and begin your sequencing.
The system will perform a quick pre-run check
to ensure success.
While your samples are being sequenced,
you can follow up with your data in real-time.
It's here where you will notice the similarities
to previous workflows on other Illumina sequencers.
From preparing samples for clustering
to the intuitive user interface,
both experienced NGS users
and those that are new to NGS technology
will find the iSeq 100 to be a refreshingly simple solution.
A big advantage to using one of Illumina's sequencers,
is the capability
of streaming and analyzing data on BaseSpace.
If you don't have a BaseSpace account,
you can sign up for one on the Illumina website
by clicking the MyIllumina tab
at the top right of the screen.
Once you have an account,
it is possible to monitor ongoing runs
on any of our sequencers.
You can analyze data
using one of the many apps available in the app store,
access public data from previous runs,
or further analyze data using BaseSpace Sequence Hub.
In addition you can perform analysis on your system directly
with Local Run Manager.
You've now seen how easy it is
to use the iSeq 100 system, from set up to analysis.
- We are excited here at Illumina
to bring a new partner into your lab, the iSeq 100,
which makes NGS sequencing more accessible than ever.
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