Welcome to Applied Biological Materials live-action video series
Do you perform a lot of Plant PCR?
We're going to show you how you can prune down your PCR workflow
with our time-saving partner, the Plant Ex-Amp PCR Kit.
All you need is 15 minutes
to extract DNA from even the most difficult pine-tree-like samples
and get yourself set-up for PCR.
This kit contains our Kodaq Polymerase
to help you tackle tough templates
like plant DNA for a highly successful PCR.
Why don't we try using the kit together?
First, let's see what's inside our Plant Ex-Amp Kit.
Some Kodaq 2X PCR MasterMix with dye,
Plant Ex-Amp Extraction Buffer,
and a 10X Plant PCR Enhancer.
You'll also need some nuclease-free water,
and some leaf samples -- we've got three!
Are you all set up?
Let's get started!
First, we'll label three eppendorf tubes with our leaf specimens
and one tube as our NTC
which is going to be our non-template control.
Now, we'll put 3 x 3 milimeter samples
of each leaf into their respective tubes
and nothing into the NTC tube.
For the next step, make sure you are in a nuclease-free environment
using a clean automatic pipettor
with aerosol resistant barrier tips
add 100 microliters of room temperature Plant Ex-Amp Extraction Buffer
to all four tubes.
Vortex everything for about 1 minute.
All that's left now is to incubate the mixtures for 15 minutes
at 95 degrees celsius.
Excellent!
You're done!
See how fast extraction was?
You can store your extracted gDNA
at -20 degrees celsius
until you are ready for downstream processing.
If you've got a difficult sample to deal with
simply perform additional freeze-thaw and vortexing cycles
and you should be ready to go!
PCR is now as simple as
preparing the premix containing your primer and our Kodaq 2X PCR MasterMix with dye,
adding your gDNA,
loading everything into PCR strips,
and running it on the PCR machine along with your NTC solution.
If you have weak or difficult amplifications
you can also add our 10X Plant PCR Enhancer
into your premix.
Perfect!
Let's go and analyze the amplified products
using agarose gel electrophoresis.
Running a gel is convenient and easy
thanks to our Kodaq 2X PCR MasterMix with dye
This MasterMix helps you to track the progress of the gel
as it runs
safely avoiding under- or over-running of your gel.
Let's see how our gel turned out!
Great!
As you can see, with our Plant Ex-Amp PCR Kit
we were able to amplify segments of the 28S
sequence in the 3 plant leaves we started with.
We hope this demonstration showcases why the Plant Ex-Amp
PCR Kit is a lab favorite for our plant scientists.
For more information on any of our PCR Kits
please click the link in the description below.
All the products used in this demonstration
can be purchased at abmgood.com
And if you enjoyed watching this video
Check out our other video series
and subscribe to get the latest updates.
Thank you for watching!