Welcome to Addgene and our video tutorial
showing you how to deposit plasmids.
I'm Quintin, and I'll guide you through the deposit process
step by step.
At the end of this video
you'll be able to get started right away
with you first deposit.
And you'll see...
depositing with Addgene is easy and free.
The depositing process begins with providing the plasmid information.
You can go to addgene.org,
click "deposit,"
then click "online submission,"
to get started.
Or, you can click "spreadsheet submission,"
download a spreadsheet,
fill in the plasmid information,
and email us as "deposit@addgene.org."
You can also email us with questions, or if you need any help.
We welcome any plasmid that's useful to other scientists.
Published,
unpublished,
or pre-publication
We can even keep your plasmids unavailable,
until your article is ready to publish.
After we've received the plasmid information,
Addgene will coordinate the execution
of the material transfer agreement, or MTA,
According to the legal process that's in place at your institution.
As soon as the material transfer agreement is finalized,
We'll send you a deposit kit,
which is a plastic box to protect your samples,
barcode stickers,
and an envelope that is prepaid by Addgene.
All we need from you is about 50 μl of DNA,
miniprep concentration and quality is fine.
Simply attach the barcode sticker to the tube,
place it in the plastic box,
and use the envelope to send the samples to Addgene, free of charge.
Once we receive your plasmid samples,
we'll do quality control that includes full plasmid sequencing.
We'll be in touch with you to confirm the sequencing results.
You can track the progress of the deposit online, and see which stage it's currently at.
After quality control is completed, your plasmids are available on Addgene's website.
When you log in to your personal depositor account,
you can see all your plasmids deposited at Addgene.
You can use this page as your lab's plasmid database,
to track requests,
or view your reward points.
Your plasmids can easily be found by other scientists on Addgene's website
under your name.
A link to your personal Addgene webpage
makes it easier for your colleagues to find your plasmids.
It's also possible to use search for the plasmid name,
or elements within the plasmid
Each plasmid page provides useful information,
such as the depositing scientist's name,
the publication,
the full plasmid sequence,
the plasmid map,
and how this plasmid should be cited when used by a requesting scientist.
If your plasmids are used in other publications, we'll add citation links.
There are a great deal of benefits when that come with depositing.
Save time and money,
because Addgene is doing the shipping of plasmids for you.
Stay organized,
All your plasmids are kept securely, and no information is getting lost.
Increase citations,
Scientists will find your plasmids easily,
and cite your work.
Receive reward points when you plasmid gets requested.
And finally, give back to the scientific community.
In the past, you may have used plasmids from your colleagues
which helped you advance your projects.
Give something back,
and feel great about it!
By becoming an Addgene depositor, you're sending a strong signal
that you care about important initiatives,
such as open science,
and scientific reproducibility.
Thank you for watching our tutorial about depositing plasmids.
Addgene's mission is to accerlerate science,
by facilitating sharing,
and giving access to authenticated research reagents.
Please contact us at deposit@addgene.org to get started,
or if we can help with any questions.
Addgene: a better way to share science.