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Hello, my name is German Gomez and I work at Restek in the Research and Development team
and I’m going to present my poster “Rapid Screening and Confirmation of Target Analytes
in Biological Fluids Using a Single Sample and a Single Sampling Collection Device”.
There is need for mass spectrometry based analytical workflows
that facilitate rapid screening and quantitation of target analytes in biological fluids.
In an ideal world, the same sample can be used for both screening and confirmation.
However, in most cases, two independent samples and sample-prep workflows
are needed to isolate the target analytes for the screening, quantitation and confirmation steps.
Herein, we present a technology that can be used to capture
target compounds from diverse biofluid samples
and perform rapid screening and quantitation via direct-to-MS experiments.
In addition, the same device can be used for confirmation of said analytes
via LC-MS/MS without further sample preparation.
This novel technology, known as Coated Blade Spray or CBS,
provides reliable and reproducible results and with a total analysis time
under 5 minutes for the rapid screening protocol.
So, what is Coated Blade Spray?
Coated Blade Spray is an SPME-based analytical technology
that facilitates collection of analytes of interest
from a sample and the direct interface to mass spectrometry systems
via a substrate spray ionization.
The device comprises a thin-flat sheet with a pointed tip and it is manufactured
of a conductive substrate such as stainless steel.
As an SPME device, the substrate is partially coated
with an extraction phase comprised of polymeric particles and a binder.
The function of the polymeric particles is to enrich the analytes of interest from the sample matrix,
while collecting the least amount of interferences.
As a direct to MS technology,
the device requires a pre-wetting of the extraction material
so to elute the analytes collected on it.
Subsequently, a differential potential is applied between the non-coated area of the substrate
and the inlet of the MS system generating an electrospray at the tip of the CBS device.
CBS devices coated with HLB particles were used in this work
and the CBS analytical protocol comprised three steps:
First, Analyte-enrichment, by extracting from a vial containing the sample of interest.
Second, Coating-cleaning, which involved immersing the CBS device in a vial with water
for fast removal of any loosely attached matrix components;
and third, Instrumental analysis,
which is performed by applying 10 µL of methanol/water onto the coated area.
After 10 seconds, 4 kV were applied to the non-coated area of blade for 5 seconds,
inducing electrospray ionization.
For confirmatory purposes, after running the direct-to-MS analysis,
the same CBS device was immersed on a vial containing
200 µL of a methanol:water solution for 5 minutes.
1 µL of this solution was injected onto the LC-MS/MS system
and analytes were separated under 3 minutes with a Raptor Biphenyl
30 x 2.1 mm column with 1.8 µm particles.
As can be seen in figure 1, excellent linearity,
precision and accuracy were obtained for different drugs tested such as cocaine,
methadone, PCP and JHW-015 spiked in oral fluids from 1-200 ng/mL.
As expected,
CBS proved to be a great tool for rapid screening and quantitation of drugs of abuse in oral fluids.
While rapid screening is ideal for most applications;
confirmatory results with a gold-standard technique is ideal.
To the best of our knowledge,
this is first time that a device that was used for ultra-fast screening
can also be used for confirmatory purposes.
Figure 2, for example, portrays the chromatogram of several analytes with diverse physicochemical properties
that were extracted with the CBS devices from the oral fluid sample.
It is important to highlight that, although is not presented in the slide,
identical quantitation results were obtained with direct-to-MS approach
and the traditional LC-tandem mass spectrometry approach.
In this work, we present the first sampling device that can be used for both
rapid screening and quantitation and confirmation and quantitation without further sample preparation.
The optimized direct-to-MS method demonstrated outstanding precision
with relative standard deviation <10 %, limits of quantitation equal to or below
5 ng/mL and excellent linearity with correlation coefficients >0.99
for each of the selected compounds over the range evaluated between 1-200 ng/mL.
Thank you for watching my poster. If you would like more information about coated blade spray
please see the link below and follow us on twitter.
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