Hi everybody, my name is Raven Baxter and you’re watching
Addgene’s Intro to the Lab Bench.
This is a safety protocol for Biosafety Levels one and two,
known as BSL 1 and BSL 2.
There are four biosafety levels, and the purpose of the four levels
is to distinguish between different hazards, and attention to protective
measures for each level,
as each level has different safety requirements.
BSL 1 is designated for those who are working with microbes
that don’t cause disease in healthy humans,
and BSL 2 for labs that work with pathogens including organisms such
such as Staphylococcus aureus or Vibrio cholerae, for example.
BSL 2 includes all of the precautions needed in BSL 1,
along with additional precautions to prevent injuries, ingestion,
and exposures to hazardous materials.
Let’s start by discussing BSL 1 guidelines.
Right after you enter the lab, you must make sure you put on your
Personal Protective Equipment (also known as PPE),
and wear it the whole time you’re in the lab.
You’ll need a lab coat
closed-toed shoes
gloves
eye protection such as safety glasses, goggles or a face shield.
We’ll talk about other things you may need throughout the video.
Do not eat,
drink
chew gum
or even apply makeup in the lab.
Before you get started with your experiment, make sure your
workspace is free of clutter.
Wipe down your space with a disinfectant, like 70% ethanol,
or a 10% bleach solution.
and that you have all of the materials you need.
You should also know about some of the items that should be present
your lab for your safety
For example, you may accidentally come in contact with hazardous materials.
In your lab there is a sink,
an eyewash station,
a safety shower,
a fire blanket,
nd an extinguisher present in the room.
and should be used in case there are emergencies.
Make sure you know where all these items are before you get started.
You should use the eyewash station
if you get something in your eye.
Make sure you wash your hands before and after working in the laboratory.
And also be sure that you know where
the designated chemical waste accumulation sites are in your lab.
There’s nothing like starting an experiment and producing waste,
and then having it like, “OK what do I do with this?” You need to be sure
you know where to put these things before you get started.
If you haven’t received hazardous waste safety training,
you absolutely should ask for it.
Make sure that you receive that training, and then
also consult the Material Data safety sheets (MSDS)
prior to working in the lab, or using chemicals you are not familiar with.
in the lab you’re working with things like glass beakers, flasks, needles.
these things, when they break or are used, can be considered sharps.
and should be placed into a sharps container.
Sharps containers are thick walled, and must be impenetrable
by a needle, and they should be able to be closed securely.
There may be times when you’re working with biohazardous
materials, and it’s super important that you
label these materials as biohazardous.
Any waste that is biohazardous, or any objects that have come into
contact with biohazardous waste, should be disposed of
in biohazardous containers.
These biohazardous materials are usually decontaminated prior
to disposal, most likely in an autoclave.
Only mechanical pipetting should be done in the laboratory.
Please do not use your mouth to pipette in the laboratory.
Make sure you clean any spills immediately,
and decontaminate as necessary.
For large spills that cannot be cleaned easily,
make sure you evacuate and close off the area,
and alert the chemical hygiene officer.
Now we’re ready talk about Biosafety level 2 Guidelines.
for working in the lab.
BSL 2 guidelines are expected to be followed
in addition to BSL 1 guidelines discussed previously in the video.
BSL 2 labs must be clearly marked as BSL 2,
and the names and contact information
of the laboratory manager should be clearly visible in the lab.
BSL 2 centrifugation steps require the use of an aerosol-tight lid,
and the rotors should be loaded and unloaded in a biosafety cabinet.
For BSL 2 labs specifically, you will need to receive
bloodborne pathogens training.
It is strongly recommended that anyone involved in BSL level work
receives a hepatitis B vaccination or titer prior starting work in the laboratory.
As discussed previously, with BSL 1 labs, it’s important to understand
how to dispose of biohazardous waste properly. In BSL2 labs,
for some biohazardous waste, an autoclave or other
decontamination methods must be used for proper disposal.
Liquid BSL 2 waste can be decontaminated in a
final concentration of 10% bleach for 30 minutes,
before pouring down the drain.
Solid BSL 2 waste can be collected and designated in biohazardous
waste containers that can be autoclaved.
Though this all sounds simple and straightforward, following the
appropriate BSL 1 and 2 safety protocols is so important,
and it's the best way to ensure you’re doing your best to
prtoect yourself while you’re doing your work.
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