(English captions by Andrea Matsumoto, University of Michigan.)
These are the items we require in the stool concentration technique.
We need the stool sample, the fine mesh of pore size three fifty to four fifteen micrometer,
the fifteen mL centrifuge tube, a glass applicator for emulsifying the stool sample, the beaker
for the filtrates, the ten percent Formol-saline for mixing the stool, then the diethyl ether.
Add ten percent Formol-saline to the stool, about a gram of the stool sample.
Emulsify the stool sample with the applicator.
Emulsify it very well to get a smooth substance.
Sift the stool sample through the fine mesh into the beaker.
Discard the stool sample.
The debris is discarded under the tap water because it has been disinfected already with
the Formol.
Transfer seven mils of stool filtrate into the fifteen mil centrifuge tube.
Rinse whatever is left in the beaker into the centrifuge tube.
Add three mils of the diethyl ether to the seven mil of the stool filtrate in the fifteen
mil centrifuge tube making it to a total of ten mL.
And we will see two layers: the ether layer and then the Formol-saline layer.
Cover the centrifuge tube with a lid and mix the two layers very well.
And thus the ether is to dissolve in the fat, which is present in the stool sample, to release
the parasites.
Put it in the centrifuge tube balancing it.
Centrifuge at thousand five hundred (1500) RPM for two to five minutes.
Okay take out the centrifuge tube and you will see four layers.
The first layer is the ether layer, the debris layer, which is the insoluble pad, and then
Formol-saline layer, and then the sediment which contains the parasite.
Break through the debris layer and discard the first three layers under the tap water,
which have already been treated with Formol.
So the sediment will contain the parasites of the egg or the larvae and then cysts were
suspended with part of the Formol-saline or saline.
Use a disposable pipet to transmit it very well and transfer a drop onto the microscope
slide.
Cover the drop of smear with the cover slip and examine it under the microscope using
the times ten objective (10X).
Low light allows better to see the parasite of the egg, the cyst, and the larvae.
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