After being done with glycolysis and the Krebs
Cycle, we're left with 10 NADHs and 2 FADH2s.
And I told you that these are going to be used in the
electron transport chain.
And they're all sitting in the matrix of our mitochondria.
And I said they're going to be used in the electron transport
chain in order to actually generate ATP.
So that's what I'm going to focus on in this video.
The electron transport chain.
And just so you know, a lot of this stuff is known.
But some of the details are actually
current areas of research.
People have models and they're trying to
substantiate the models.
But things are happening at such a small scale here that
people can just look at the evidence, some of which is
indirect, and say, this is probably what's happening.
Most of this is very well established, but some of the
exact mechanisms-- for example, how exactly some of
the proteins work-- aren't completely understood.
So I think it's very important for you to understand that
this is at the cutting edge, that you're already there.
So the basic idea here is that the NADHs-- and that's where
I'll focus.
FADH2 is kind of the same idea.
Although its electrons are just at slightly
lower energy state.
So they won't produce quite as many ATPs.
Each NADH is going to be-- as you'll see-- indirectly
responsible for the production of three ATPs.
And each FADH2, in a very efficient cell, in both of
these cases, will be indirectly responsible for the
production of two ATPs.
And the reason why this guy produces fewer ATPs is because
the electrons that he has going into the electron
transport chain are at a slightly lower energy level
than the ones from NADH.
So in general, I just said indirectly.
How does this whole business work?
Well in general, NADH, when it gets oxidized-- remember,
oxidation is the losing of electrons or the losing of
hydrogens that happen to have electrons.
We can write its half reaction like this.
Its oxidation reaction like this.
You'll have some NAD plus, which you can then go and use
back in the Krebs Cycle and in glycolysis.
You have some NAD plus, you'll have a proton, a positive
hydrogen ion is just a proton.
And then you'll have two electrons.
This is the oxidation of NADH.
It's losing these two electrons.
Oxidation is losing electrons.
OIL RIG.
Oxidation is losing electrons.
Or you can imagine it's losing hydrogens, from which it can
hog electrons.
Either one of those is the case.
Now this is really the first step of the
electron transport chain.
These electrons are transported out of the NADH.
Now, the last step of the electron transport chain is
you have two electrons-- and you could view it as the same
two electrons if you like-- two electrons plus two
hydrogen protons.
And obviously if you just add these two together, you're
just going to have two hydrogen atoms, which is just
a proton and an electron.
Plus one oxygen atom, so I could say one half of
molecular oxygen.
That's the same thing as saying one oxygen atom.
And you're going to produce-- if I have one oxygen and two
complete hydrogens, I'm left with water.
And you could view this, we're adding electron or we're
gaining electrons to oxygen.
OIL RIG.
Reduction is gaining electrons.
So this is the reduction of oxygen to water.
This is the oxidation of NADH to NAD plus.
Now, these electrons that are popping out of-- these
electrons right here-- that are popping out of this NADH.
And when they're in NADH they're at a
very high energy state.
And what happens over the course of the electron
transport chain is that these electrons get transported to a
series of, I guess you could call
them transition molecules.
But these transition molecules, as the electrons go
from one to the other, they go into slightly
lower energy states.
And I won't even go into the details of these molecules.
One is coenzyme Q, and cytochrome C.
And then they eventually end up right here and they are
used to reduce your oxygen into water.
Now every time an electron goes from a higher energy
state to a lower energy state-- and that's what it's
doing over the course of this electron transport chain--
it's releasing energy.
So energy is released when you go from a higher state to a
lower state.
When these electrons were in NADH, they were at a higher
state than they are when they bond to coenzyme Q.
So they release energy.
Then they go to cytochrome C and release energy.
Now that energy is used to pump protons across the
cristae across the inner membrane of the mitochondria.
And I know this is all very complicated sounding.
And this is the cutting edge.
So it maybe should sound a little complicated.
Let me draw a mitochondria.
So let me draw a small mitochondria just so you know
where we're operating.
That's its outer membrane.
And then its inner membrane, or its cristae,
would look like that.
And let me zoom in on the membrane.
So let's say if I were to zoom in right there.
So if I were to zoom that out, that box would look like this.
You have your crista here.
And I'm going to draw it thick.
So I'm zooming in.
This green line right here, I'm going to
draw it really thick.
I'm going to color it in with the green, just like that.
And then you have your outer membrane.
This outer membrane, I can do it up here.
And I'll just color it in.
You don't even have to see the outside of the outer membrane.
Right here, this space right here, this is the outer
compartment.
And then we learned in the last video, this space right
here is the matrix.
This is where our Krebs cycle occurred.
And where a lot of our NADH, or really all of
our NADH, is sitting.
So what happens is, every time NADH gets oxidized to NAD
plus, and the electrons keep transferring from one molecule
to another, it's occurring in these big protein complexes.
And I'm not going to go into the details on this.
So each of these protein complexes span-- so let's say
that's a protein complex where this first oxidation reaction
is occurring and releasing energy.
And then let's say there's another protein complex here,
where the second oxidation reaction is occurring and
releasing energy.
And these proteins are able to use that energy to essentially
pump-- this might all seem very complicated-- to
essentially pump hydrogens into the outer membrane.
It actually pumps hydrogen protons.
And let me be very clear.
Hydrogen protons into the outer membrane.
And every one of these reactions pump out a certain
number of hydrogen protons.
So by the end of the electron transport chain, or if we just
followed one set of electrons, by the time that they've gone
from their high energy state in NADH to their lower energy
state in water, by the time they've done that, they've
supplied the energy to these protein complexes that span
our cristae to pump hydrogen from the matrix
into the outer membrane.
So really the only byproduct of the oxidation of NADH into,
eventually, water, or the oxidation of NADH and the
reduction of oxygen into water, isn't ATPs yet.
It's just this gradient where we have a lot higher hydrogen
proton concentration in the outer compartment than we do
in the matrix.
Or you could say that the outer compartment becomes a
lot more acidic.
Remember acidity is just hydrogen proton concentration,
the concentration of hydrogen protons.
So the byproduct of all of this energy is used to really
just pump these protons into the outer membrane.
So you have two things.
The outer membrane becomes more acidic
than the matrix inside.
Maybe we could call that basic.
And obviously these are all positively charged particles.
So there's actually an electric gradient, an electric
potential between the outer membrane
and the inner membrane.
This becomes slightly negative, that becomes
slightly positive.
These guys wouldn't naturally do this on their own.
If this is already acidic and it's already positive, left to
its own devices, these more protons wouldn't be entering.
And the energy to do that is supplied by electrons going
from high energy state in NADH to going to a lower energy
state, eventually, on the oxygen in the water.
That's what's happening.
But essentially all that's happening is protons being
pumped from the matrix into the outer compartment.
Now once that gradient forms, these guys
want to get back in.
These guys want to get back into the matrix.
And that is where the ATPs are formed.
So there's a protein that also spans this.
Let me draw.
Remember this is all this inner membrane right here.
Let me just draw it a little bit bigger right here.
So that's our inner membrane, our cristae right there.
There's a special protein called-- and I'll show you
actually a better diagram of what looks like in a second--
called ATP synthase.
And what happens is, remember because of the electron
transport chain, we have all of these hydrogen ions up
here, all of these protons really.
All they are is a proton.
That really want to get back into the matrix down here.
But they can't.
This crista is impermeable to them so they have to find a
special way to get through.
They were able to go the reverse direction through the
special protein complexes.
Now they're going to go back into the
matrix through ATP synthase.
So they're going to go back, but
something interesting happens.
And this is really an area of current research where people
think they know how it works but they're not sure.
Because you can't just take these proteins apart and watch
them operate like you can a regular mechanical engine.
These are ultra-small and they have to be in a living system.
And they have to have the right conditions.
And you can't even-- it's hard to see hydrogen protons.
These are ultra-small things that are, pretty much, you
can't see them.
But what happens, the current model is, as these enter, as
these go through my ATP synthase
there's actually an axle.
So you can kind of view this as a housing.
And then there's an axle.
And this is all just a big protein.
And there's an axle and then there's another part of the
synthase down here.
So you can imagine, this is kind of mind-blowing.
That something this fancy is occurring on the membranes of
pretty much all living systems' cells.
It's not just eukaryotes.
Even prokaryotes do this.
They don't do it in their mitochondria; they do it in
their cellular membrane.
But it's a pretty neat thing.
And what happens is, as these go through, you can kind of
imagine as water flowing through a turbine.
It mechanically causes this structure in
the middle to spin.
To actually spin.
This is the current thinking.
And this thing is all uneven.
It's not, like, this nice tube.
It'll look all crazy like that.
And what happens is that an ADP molecule-- let's say that
this is the A part of the ADP.
And then you have two phosphate groups.
It'll attach to one part of this protein.
And maybe a phosphate will just randomly attach to
another part of this protein.
Just like that.
So right now it's just ADP and a phosphate.
But as this inner axle turns-- because it's not a symmetrical
tube, it has different things sticking out that have
different amounts of atomic charge and it's going to play
with this outer housing right here.
And so as this turns, the outer housing, because of just
the proteins bumping against each other and electrical
charge and whatever else, it's going to squeeze the ADP and
the phosphate together to form, actually form, ATP.
And actually the current thinking is that it does it on
three different sites simultaneously.
So as this spins around, ADP and phosphate groups kind of
show up on the inside of this housing.
You could imagine it like that.
And I don't even know if it's on the inside.
But they show up on the housing.
And as this thing spins around, it stretches and pulls
on this outer part and pushes these two things together.
So it's using the energy from this proton gradient
to drive this axle.
And because it's all strange, it does all these distortions
on this outer part and actually pushes
the two ATPs together.
So when you start off with your 10 NADHs, it'll provided
just enough energy and just enough protons to put into the
outer membrane that when they go back through our ATP
synthase-- you could almost view it as an ATP synthase
motor-- just based on people's observations they see that
this will produce, on a per-NADH level,
roughly three ATPs.
On a per-FADH2 level, roughly two ATPs.
And I've said multiple times in the videos, this
is kind of an ideal.
That a lot of times, maybe you'll have some protons leak,
so their energy can't be captured properly.
Or maybe some of these electrons might somehow jump
the gun or jump some steps, so some of the energy gets lost.
So you don't always have a completely efficient system.
And just so you believe that this is actually occurring on
our membrane, there's actual visual
depictions of these proteins.
This is the actual protein structure of ATP synthase
right here.
That is actually ATP synthase.
And as you can see, there's this piece right here that
holds this part and that part.
You can kind of imagine it relatively stationary.
The hydrogen comes through here.
The axle gets spun.
And as the axle gets spun, ADP and phosphate groups that are
lodged inside this F1 part of the
protein, get pushed together.
You have to put energy into the reaction in order to make
them stick together.
But they get pushed together by the protein itself as this
axle turns around.
And this axle turns around from the energy of the
hydrogen going.
I don't even know what the mechanics would look like.
But you could imagine-- in my head I imagine, the simplest
thing is a windmill.
Or not a windmill, as maybe some type of water turbine or
maybe the simplest thing is, if you have
something like that.
I don't know if that's what the protein
actually looks like.
If you have any kind of thing passing by, it's
going to spin it.
It's going to spin it like that.
And you could be more creative if you want to change the
angle of the spin and whatnot.
And that's all, people are really still trying to
understand this at a deeper and deeper level.
But for your purposes, especially in an introductory
biology level, you just have to realize that two things are
happening in the electron transport chain.
Electrons are moving from the NADHs and the FADH2s to
eventually show up and reduce the oxygen.
And as they do that, they're releasing energy as they go
from one molecule to another.
They're going to lower energy states.
That energy is used to pump hydrogen protons into the
outer compartment of the mitochondria.
And then that gradient, those hydrogen protons want to get
back into the matrix of the mitochondria.
So as they go back in, that drives this ATP synthase
engine, which actually produces the ATP.
So just like we said in the past, when you have 10 of
these, on average-- let me say this way-- on average each
NADH is going to produce 3 ATPs.
Not directly.
It produces enough of a gradient of hydrogen protons
to produce 3 ATPs in the ATP synthase.
And each FADH2, on average, produces enough of a hydrogen
gradient to produce 2 ATPs.
So if we come in with 10 NADH, they're going to produce-- in
this ideal world-- 30 ATP.
And then our 2 FADH2s are going to produce 4 ATP.
And then if you remember from glycolysis, we had 2 net ATPs
directly produced.
And from the Krebs cycle we had 2 ATPs directly produced.
So then you have 4 from glycolysis and Krebs, and that
gets us, once again, to our magic 38 ATPs from one
molecule of glucose.
And now, I think you have a pretty good grasp of cellular
respiration.