the Sartorius label-free protein analysis portfolio has just expanded
in addition to our innovative industry standard fluidics-free bio-layer interferometry technology
we have now added the first Octet surface plasmon resonance instrument the Octet SF3 SPR
combining many of the features that researchers expect from bli technology like accuracy
precision ease of use and simple maintenance the Octet SF3 offers a robust high sensitivity
high throughput SPR alternative the Octet SF3 is prepared for whatever challenge you take on
making use of a range of powerful attributes including a sensitivity of less than 0.025 ru
a 768 well sample capacity more than 72 hours of unattended runtime flexible injection volumes and
extended dissociation times the power of the Octet SF3 also lies in its diverse range of injection
types from industry standard multi-cycle kinetics to the patented one step one step two comp one
step high throughput one step pulse and next step gradient injections one step gradient injections
are capable of creating an analyte gradient of at least three orders of magnitude this is achieved
by diffusing a single analyte concentration into a moving stream of buffer which removes the need
to create multiple dilution series this means you no longer need to spend time preparing multiple
dilution series or worrying about inaccuracies in creating a specific analyte concentration series
instead one step gradient injections enable an accurate and comprehensive measurement
of a molecule's kinetics and affinity from a single analyte concentration in a single well
this means that analysis of a 96 well sample plate really does generate comprehensive data
for 96 different samples imagine screening 768 unique compounds
in a single unattended run with no differences in results compared to multi-cycle kinetics
irrespective of the analyte concentration used after rapidly screening for molecules which
warrant further investigation it's also important to understand their behavior across a range
of different conditions one step two component gradient injections helps you do this seamlessly
an alternative buffer analyte or competitor molecule is injected immediately before your
primary analyte allowing you to systematically assess various physiological conditions
this can include varying ph or chemical composition during real-time binding or the
rapid screening of multiple buffers and compounds all achievable with a single running buffer
and because samples can vary in size shape and structure their behavior under a range of
conditions is also likely to differ considerably one-step pulse injections can rapidly determine
the diffusion coefficient and overall aggregation from as little as five microliters of your sample
providing accurate empirically derived results instead of having to rely on calculated
theoretical coefficients competition assays are a critical component of the drug discovery process
our next step injection technology is a competitive injection type that focuses on
speed and throughput since the competitor molecule isn't diluted with the running buffer multiple
competition assays with different competitor molecules can be performed against one analyte
from a single unattended run this also saves significant quantities of each competitor molecule
and to complete the package an intuitive user-friendly acquisition and analysis platform
is essential the high-quality data captured with the Octet SF3 is easily analyzed using the
octet spr discovery software with its intuitive drag and drop layout users can rapidly define
sample parameters create common or independent sample flow rates add injection volumes and select
from numerous pre-written methods to help guide their setup whatever your project assay compound
or biologic of interest the Octet SF3 is powered and prepared for whatever challenge you take on