(light music)
- Hi, my name is Wendy and I'm an applications trainer
here at Illumina.
I'm here to give you a brief introduction
to Illumina's newest prepared sample to analysis solution
for amplicon sequencing, AmpliSeq for Illumina.
AmpliSeq for Illumina is our newest library prep solution.
The AmpliSeq line leverages the ease of use
and expedient AmpliSeq workflow many customers
are already familiar with, to create targeted libraries.
Combined with the scalable sequencing power
of Illumina's platforms and push button bioinformatics,
targeted sequencing has never been so accurate or so easy.
AmpliSeq for Illumina encompasses a multitude of content
that can be divided into three basic buckets:
Ready to use DNA Panels, Ready to Use RNA Panels,
and Custom Panels.
For customers who prefer pre-designed content,
there are a number of panels available
for different disease areas.
For customers who prefer a more custom approach,
panels can be easily designed anew or assembled
from pre-existing content using our DesignStudio software.
To access the tool, a researcher would simply go
to the Illumina website and sign in
using their MyIllumina account.
The Illumina DesignStudio designer is a quick
and intuitive tool for custom panel design.
With the DesignStudio tool, users can personalize content
to fit their experimental needs.
Once inside the design tool, the user would
select Start Design, choose the desired input and species,
select an amplicon size, and proceed to enter gene names
or coordinates for each target of interest.
When finished, select the Submit for Design button.
Finalized designs are usually ready within a few hours.
For those who aren't sure what genes or coordinates
to cover, we have a variety of pre-designed panels,
tested and optimized in our own labs.
AmpliSeq for Illumina Community panels, are also available.
Community panels are composed of content assembled
by researchers and collaborators in different application
areas that have been made available to the wider public.
Once the hard work is over and the panel has been ordered,
it's time for the fun part.
What I'm most excited about is the simplicity
of the AmpliSeq process.
Once your DNA or cDNA has been quanted and is ready to go,
you are ready to start the AmpliSeq for Illumina workflow.
Starting with as low as 1ng of DNA or cDNA input
for most panels, in less than 6 hours
you can have ready-to sequence libraries.
Let's take a closer look.
Once your DNA has been quanted, or your RNA has been quanted
and converted to cDNA, you are ready to begin
the AmpliSeq for Illumina workflow.
Starting with as low as 1ng of either DNA or cDNA input,
using either a fixed or custom panel primer pool,
prepare your PCR reaction.
In the first step of the workflow, multiplex PCR is used
to amplify your target regions of interest
and create amplicons.
In the second step of the workflow, you take these amplicons
and perform a partial digestion to eliminate
the overhanging primer ends and blunt end the amplicons
in preparation for adapter ligation.
In the third step of the workflow, these blunt ended
fragments are ligated to Illumina index adapters
to allow for multiplexing and sequencing.
Targeted AmpliSeq for Illumina panels, although compatible
with all Illumina sequencers, tend to be sequenced
more frequently on our benchtop platforms.
With a smaller amount of target content,
benchtop instruments provide the right balance
between coverage depth, cost, and sample number per run.
Examples of Illumina benchtop instruments include
the MiniSeq, MiSeq, and NextSeq.
Our benchtop systems combine a small footprint
and a user friendly interface with the power and accuracy
of SBS chemistry, the most widely adopted
sequencing technology in the world.
But what is sequencing data, without an analysis solution
to help researchers make sense of it all.
For AmpliSeq for Illumina panels, we have several
flexible analysis solutions to allow for data crunching
on or off instrument using either Illumina's Local
Run Manager software, or up in the cloud
using BaseSpace Sequence Hub.
With Illumina analysis solutions, you don't need
to be a bioinformatics expert or maintain
complicated computer infrastructure.
The Illumina analysis solution supports different workflows
for analysis, the DNA Amplicon Analysis workflow
and the RNA Amplicon Analysis workflow.
With DNA amplicon panels, the analysis workflow aligns reads
against the reference genome and calls small variants
and indels.
With RNA amplicon panels, the analysis workflow performs
differential expression analysis and gene fusion calling.
These analysis workflows generate output files
called variant call files or .vcfs that can be used
in downstream analysis.
For an even deeper biological view, these variant call file
outputs, or .vcf files, can be uploaded into Illumina
BaseSpace Variant Interpreter software.
By cross-referencing the variants in your sample against
a large collection of annotation databases,
users can rapidly identify relevant variants
and perform annotations in an easy to read reporting format.
Together, AmpliSeq for Illumina library preparation
and the power of Illumina SBS sequencing offers
a highly scalable, and confident approach
to targeted resequencing.
Now you can experience targeted resequencing, amplified.
For more information on AmpliSeq for Illumina products
and workflows, visit the AmpliSeq product and support pages
at www.illumina.com.
And as always, if you have any questions, please feel free
to reach out to Illumina Technical Support.
Scientists just like you are available and happy to help.
Best of luck experimenting!