[Scott Robinson] All right! So we're going to go to we're going to go backwards we're
going to go as far as them as the suite goes in this direction then we're going to
go back to the main part of the suite so the space that we're entering now
used to be a clean room we completely reconfigured it we can work with live cells in here this is
biosafety level 2 compatible space we're also set up to do a cell culture if we need to
mostly it's people keeping their cells alive so they can use this instrumentation
a couple of incubators in here bio safety hood refrigerator and then this is the uh
this is like sp8 we don't see it right now but it has an oco labs incubator on it so that you
hook that up to uh CO2 and you have a very small incubator about the size of a cigarette pack
you can keep yourselves alive then this next room we're just going to buzz through it really quick
this is where we're set up to do uh forster resonant energy transfer imaging it's also
set up for turf total internal reflectance fluorescence and we have a super brilliant
light microscopic spectroscopist who built this so this is set up with uh lasers really does a
beautiful job doing turf you know and terp is one of the super resolution uh microscopy deals where
you get about 200 nanometers of super resolution imaging so it's used for looking at membranes
and things that are really close to this cell membrane just through a super thin uh coverslip
the space we're in now
was built for a guy who wants to do two-photon multi-photon on live rats what we did is we made
we made us built a soundproof room in here when we have a two-photon multi-photon on a microscope
then you have to be a lot more careful so it has a great big enclosure around it and you're supposed
to keep everything closed while you're imaging this is a broker system this also is set up for
uh electrophysiology we're going to go into the micro ct area here in this space we have um
if we look over this way you have a ct lab 1 130 instrument and this one the sample is static other
in every other one of these systems the sample moves this is a revacu system this is a uh rigako
rugaku nano 3dx system this one gets uh i think about 170 nanometer resolution
so really pretty good resolution but when we give you these great resolution numbers uh it's tricky
to get something like that so we just roll out these numbers but it's not always as easy as it
sounds we have a next radio micro ct system you can look at insects you can look at bone this one
is also another x-radius system and we've i've set this up various ways so we've made different kind
of modifications so that you can look at things in liquids we have people who work with plants
and what happens is if you cut a leaf open it's going to bleed out so it's set up so that we'll
put that piece of a leaf inside a straw and seal it up and do that kind of work this is really a
pretty nice micro ct setup we're going to go to the main part of the suite
this is the wet lab and we have a oxygen plasma cleaner
glow discharge for tem two splutter coaters for sem it's always good to have two sputter coaters
in case you break one we have a dual metal evaporator back here so that we can coat uh
like you can put chromium on something and without breaking the uh vacuum then you can
coat with something else so that it's it's just you put about 10 or 12 angstroms of chromium on
something and then you put your other stuff on it and it actually seals better it's kind of
a lot of work for something fairly simple and then the wet lab is available to lots of people
this is just a regular fluorescence microscope so this is a zeiss then if we switch gears completely
we have a brand new uh transmission electron microscope so this is a a scanning transmission
electron microscope and stem it's a refurbished system 4k by 4k cameras it does high angle annular
dark field imaging and that's really kind of a cool thing where you can get a sort of
elemental analysis out of it because it's a stem because it's an stem you can control the beam if
you can control the beam then you can also do eds so and down the road i'll be able to do
elemental analysis using the stem system and then here this is another noisy room just to peek into
this is high pressure freezing and freeze substitution for transmission electron microscopy
so when we're working with biosamples which is what we do a lot to be able to freeze the sample
so fast that the hydrogens don't have time to swing around and we have our samples in vitreous
ice so in glassy ice and so what do you do after that so this is what you do when you do cryo
microscopy when you do cryo-em you're freezing samples in vitreous ice we're taking samples
that are a little bit larger like nematodes and stuff like that using them in vitreous
ice and then taking about six or seven days to run them through a freeze substitution process
where we can embed them in plastic from going from being frozen in ice to embedded in plastic
but the uh the substructure the ultrastructure is preserved all the way through this is uh atomic
force microscopy this is just straight up afm and then we also have this thing it looks like a
pizza oven in here is a stylus profilometer and the stylus profilometer is kind of like an afm
except it has much better amplitude so for example you can put a coin in there
and you can see the uh the surface of the coin where if you're doing afm there's no way you'd
be able to see that depth so this is a 10 cent uh euro coin from 2006 i think really pretty
and then we have over here this room is set up this is a stereology workstation
we're counting slide after slide after slide cell after software cell of brain
after brain and so they're looking at phthalates uh exposure to phthalates exposure to bisphenol a
bisphenol s all these different kinds of things it gets used a lot so we're really actually
sort of lucky we're not interrupting someone right now this other microscope in here is uh
one of our few upright microscopes it had really big stage on it and this one has dark field optics
and we also when we bought our uh ramen confocal we got a cold stage for uh light microscopy
a hot stage for light microscopy and they both conveniently fit on this
uh ins what we call an inspection microscope another thing that we got is for tension
or for compression that means that you can bend and break and squish things inside the sem and
get a close-up view of it if you want to this is a environmental scanning electron microscope which
means it's also a regular scanning electron microscope it has a field emission gun on it
and uh what nate is looking at right now is um cross-sections of nematodes
so what we're looking at here is uh is 40 nanometer thick sections that are sitting on
coated slides that have been coated with gold and palladium uh or gold yeah gold palladium and
uh nate's bringing the focus in on these so that he can relax he's using
uh back scattered imaging in order to be able to differentiate what he's looking at
so they're really really beautiful cross-sections so this also has um eds on it so you can do
elemental analysis you can work with wet samples you can work with frozen temples
uh lots of different things like that really really nice uh field emission microscope and
then dpv we got a uh hariba Raman confocal system so when the confocal Raman system which somebody
is using right now got this other system with deep uv so it's a really nice system it's also
set up for fluorescence and it also has a 120 000 frame per second camera just sitting up there
so this is just another space where we have critical point dryers and automated critical
point dryer regular critical point dryer two ultra microtomes fiber puller micro pipette puller
uh carbon evaporator just for things that we wanted to make conductive using carbon and then a
little toy uh tinsel stage in here that somebody gave us so that is really most of it uh right
where we are now we're in the basement of beckman down the hall you have spec pet ct we're looking
at live animals and there's that's a really nice setup it's called the molecular imaging laboratory
they do some really beautiful work and then if you come a little bit more this way we have two uh
MRI setups that are for people so those are right right next to us and across the hall there's also
uh sound related imaging so ultrasound things like that. So i think we're done!