So today I want to talk to you about enzymes
and how they're critically important pieces
of cellular machinery.
But first, let's review the idea that biochemical reactions
happen in the body all the time.
Almost every cellular process involves a biochemical reaction
at one point or another.
You know, the TCA cycle is actually
just a series of different biochemical reactions
in carbon metabolism.
DNA replication, which needs to happen before a cell
to go through mitosis, is also just a series of reactions.
And this also applies to the expression of genes,
going from DNA to RNA to protein.
And we need enzymes because enzymes
make all of these reactions go much faster.
And let's look at this idea little more deeply,
and how a reaction will go on differently
when it has an enzyme versus not having one at all.
So you may be familiar with the reaction
where water and carbon dioxide can
combine to form carbonic acid.
And this is a reversible reaction,
so it can go backwards and forwards.
Now, when people make soda or any carbonated beverage,
they'll start by pumping that soda can full of CO2.
And while some of that CO2 will dissolve
in the water and the can, the soda making companies
are able to get a lot more CO2 in the water
by using this reaction.
The abundant CO2 will react with the water
to form some carbonic acid in the can.
And when you go to open the can, you'll
hear a pop sound, which is really
just a bunch of CO2 escaping.
But after that, the soda will start to fizz really slowly.
And what's happening here is the carbonic acid
that was made before is slowly dissociating back
to carbon dioxide in water as CO2 escapes.
And that extra CO2 that's being made
will come out of the soda solution,
and you'll see it as little bubbles floating around.
But what happens if you then take this person over here,
and he'll pick up a can of soda and take a drink?
That person might notice the soda will
start fizzing a lot more once it hits his or her tongue.
And this is because humans have an enzyme in their blood
and saliva called carbonic anhydrase.
And this makes the carbonic acid turn
into carbon dioxide in water much more quickly.
So more CO2 will come out of the can, and it will fizz more.
And this is just one of the many examples
of how enzymes make reactions go faster.
So how exactly do the enzymes make the reactions go faster,
though?
Well, they use a bunch of different catalytic strategies
to push reactions along a little more quickly.
And I'm going to talk about a few those strategies just
to give you an idea of what enzymes are doing.
So first I'll mention acid/base catalysis,
which happens when enzymes act like either acids or bases.
Now, remember that acids and bases
are proton donors and acceptors.
And if you look at this type of reaction, which if you remember
from organic chemistry is a keto-enol tautomerization
reaction.
We have a proton moving from a carbon atom to an oxygen atom.
And since acids and bases are pretty good proton carriers,
they could both help with this reaction,
make it go a little more quickly,
by helping to move that proton around,
instead of this molecule of doing it by itself.
Our next catalytic strategy is covalent catalysis,
which happens when enzymes form a covalent bond
with another molecule, usually their target molecule.
Remember that covalent bonds involve
two molecules sharing electrons.
And looking at this reaction here,
we have a decarboxylation reaction going on.
Which, if you remember for organic chemistry,
is when a carboxy or CO2 group is being taken off a molecule.
And, if you remember, these reactions usually
have a lot of electrons moving around.
So if we had covalently bound enzyme
that could hold on to some electrons,
be an electron carrier, or what some people like
to call an electron sink, then that
would definitely help this type of reaction move a little more
quickly.
Next, we have electrostatic catalysis.
Now, if you remember, DNA is a very
negatively charged polymer because of all the
negatively charged phosphate groups that we find in DNA.
So if an enzyme had a metal cation on it, like magnesium,
we could use it to stabilize the negative charge found in DNA
and make it a little easier to work with.
And DNA polymerase, which is the enzyme that
allows DNA replication to occur, does exactly this.
And in order for it to help with DNA replication,
it needs to find a way to counteract
all of the negative charge on DNA.
The magnesium ions totally come in handy there.
So the last catalytic strategy I want to mention
is a little more general.
And it has to do with proximity and orientation effects.
Remember that in order for two molecules
to react with each other, which is usually what enzymes help
out with, they need to physically collide
at some point.
If we have molecule A and molecule B,
they'll only react once they crash into each other.
And a lot of enzymes are able to bring two molecules
close together, so that these types of collisions
happen more often, making the two molecules react
more quickly.
Also remember that the orientation
of the two colliding molecules in space
is also really important.
If molecule A and molecule B collide, but one of them
is upside down or not in the correct position,
then the collision may not result
in a successful reaction.
So enzymes also make sure that the two molecules will
collide in the right orientation.
And all of this increases the frequency of collision
in general, but also helps to make sure
that those collisions are successful
and result in a reaction.
So what did we learn?
Well, first we learned that the role enzymes play
is to make biochemical reactions happen more quickly.
And the next thing we talked about
were four of the many different catalytic strategies
that enzymes can use.
We talked about acid base catalysis
which helps with proton transfer.
We talked about covalent catalysis,
which helps with electron transfer.
We mentioned electrostatic catalysis,
which deals with stabilizing charge.
And finally, proximity in orientation effects,
which increase the frequency of successful collisions
between molecules that we want reacting together.