In this video, you'll learn how to log into your ChemiDoc
use Smart Tray Technology
and capture chemiluminescent blot images in three easy steps
and export them.
To turn on the ChemiDoc simply push the green button in front of the instrument.
When the customizable home screen appears
tap anywhere and your login menu will appear.
If you're a new user
type the desired username
click the plus sign and the new login will be entered.
Administrative controls can be set by clicking the person icon in the top right hand corner.
To begin, open the imager and pull out the transilluminator.
Note that all gels and blots will always be imaged on one of three trays
It is easy but important to clean the trays
Simply use DI water and a kimwipe to ensure optimal performance.
Smart Tray Technology automatically responds to the application-specific trays
and adjust imaging parameters and software options
For chemiluminescent blots select the UV tray.
After applying your substrate, center your membrane on the UV tray.
Once you are satisfied with the position push the transilluminator back in and close the drawer.
Zoom in until you have the desired imaging area
or simply select
small, medium or large under image size.
Tap application and select chemiluminescence under blots.
Take a preview of your image and set your region of interest.
This can be very useful for excluding abnormally bright areas that you won't want to quantify,
such as your marker.
You may choose between entering your exposure time or detecting your bands automatically.
Use optimal exposure to benefit from the full dynamic range of the instrument.
Use rapid exposure for shorter exposure times.
For low-light, quantitative assays, like chemiluminescence, additional options are available in the exposure menu.
The ChemiDoc provides a recommended binning setting
for each image but user control is enabled to adjust from 1x1 to 8x8 binning.
Select manual imaging to access Signal Accumulation Mode.
Signal Accumulation Mode makes it easy to find your ideal exposure time.
Simply enter the time you want your first image captured and the time you want your last image captured
and the number of images you wish to view.
For minimal background we recommend fewer than five images per sequence
or you can simply capture a single image by selecting set manual exposure time
Tap the camera button to capture the image.
The image that appears is automatically saved to the gallery.
To see your molecular weight marker easily capture a colorimetric image
Then, select your favorite chemi exposure
and tap merge at the bottom of the gallery screen.
You can also capture the chemi image and an image of your marker
at the same time by capturing a multi-channel image
For a quick assessment of the band intensities you can tap each band
the relative fluorescence units and percent to saturation will appear.
To adjust image appearance tap the transform icon in the lower toolbar.
The adjustments will be saved but the actual data will remain unaffected.
Your images will be saved and exported to the gallery.
Tap the image info icon.
You can enter a custom name and any notes you want to save about your image or experiment.
Tap the Send/Save icon to select the file format you want.
You can export images in multiple file types.
Under export options, tap a file type and the destination
Either saving to a USB Drive or to the network.
If you choose the image lab file format your custom name and notes as well as all the data indicated below are exported to
Bio-Rad's license free Image Lab analysis software
available for both Mac and PC.
We hope that this instructional video has showed you how to capture the
perfect chemiluminescence blot image.
For further learning use the complete, searchable
on-board manual
or the Teaches as you tap Tap software assistance tool