All cells require nutrition.
A properly formulated media contains the necessary nutrients to allow cells to grow and produce.
Yeast Extract Tryptic Soy Broth
Ammonium chloride Sodium bi-phosphate
Monopotassium phosphate and an Antifoam compound
Once all of these ingredients are in, another 10 kilograms of High Purity Water is added...all
ports and valves are closed...all condensate valves are opened...and the bioreactor begins
an SIP – Sterilize-In-Place cycle.
The target for sterilization is 121 degrees Celsius for 30 minutes.
As soon as that target is reached, the condensate valves are closed, and the SIP cycle completes
automatically.
Both the vessel and the media are now sterile…And we’re ready to add the final ingredients
to our media.
The glucose hose is attached to the vessel.
The connection is steamed to sterilize it, and the separately sterilized glucose-antibiotic
solution is pumped into the vessel.
Then a manual pH reading of the media is taken, and the bioreactor is set up for its fermentation
cycle.