In this video, you'll learn how to login to your ChemiDoc
use Smart Tray Technology
and acquire DNA gel images in three easy steps
and export them
To turn on your ChemiDoc simply push the green button in front of your instrument
When the customizable home screen appears tap anywhere and your login menu will appear
If you're a new user, type the desired username
click the plus sign and the new login will be entered
Administrative controls can be set by clicking the person icon in the top right hand corner
To begin open the imager and pull out the transilluminator
Note that all gels and blots will always be imaged on one of three trays
It is easy but important to clean the trays
Simply use DI water and a kimwipe to ensure optimal performance
Smart Tray Technology automatically recognizes the application specific trays
and will adjust imaging parameters and software options
for imaging sybr safe DNA applications
use the UV Tray or the optional Blue Tray
the Blue Tray protects samples from UV damage
it also allows you to visualize DNA samples without a UV shield
The UV Safety Shield is necessary for band excision of UV excited stains
Center your sample on the appropriate tray
Once you are satisfied with the position
Push the transilluminator back in and close the drawer
Zoom in to the desired imaging area
or you can simply select image size
Tap application to select a category and stain appropriate for your sample
If desired you may take a preview of your image and set your region of interest
This can be very useful for excluding very bright areas that you won't want to quantify
such as your marker.
Choose between entering your predetermine exposure time
or automatic detection of your bands
Tap the camera button to capture the image
For a quick assessment of the band intensities
tap each band and the relative fluorescence units as well as percent to saturation will appear.
Tap the transform icon in the lower toolbar to adjust image appearance
the adjustments will automatically be saved but the actual data will remain unchanged
Your images will be automatically saved and exported to the gallery
Tap the image info icon
Here you can enter a custom name and notes about your image or experiment
Tap the Send/Save icon to select the file format
You may export images in multiple file types
under export options tap a file type and the destination
either saving to a USB Drive or to a network
when you choose the image lab file format
your custom name and notes
as well as all the data indicated below are exported to
Bio-Rad's license free Image Lab analysis software which is available for both Mac and PC
We hope that this instructional video has taught you how to capture a nucleic acid gel image
For further learning please utilize the complete searchable on-board manual
As well as the teaches as you tap software assistant feature