The Hanna Instruments HI758 Marine Calcium Checker is a compact and portable photometer
that combines the accuracy of professional photometers with the convenience of chemical
test kits. The one button design allows users to operate the meter with ease.
Today, we will go over the proper use and technique of the HI758.
The HI758 has been redesigned to include a 0.1mL pipette which allows for more accuracy,
necessary while performing the calcium measurement.
The HI758 kit comes with everything you need to begin testing.
Two sample cuvettes with caps Reagents for 25 tests
Plastic dropper One 1mL syringe with tips
One 0.1mL mechanical pipette with tips One AAA Battery
and the instruction manual with reference guide
When you first receive your Checker, install the battery in the compartment on the underside
of the meter.
The HI758 is designed to make testing quick and easy. Press the button to power the meter
on. Notice Add C1 appears on the screen. You are now ready to begin testing.
Using the 1mL syringe, add 1mL of reagent A to the cuvette. Then, using the dropper,
fill the cuvette to the 10mL mark with deionized water.
Once the reagent and water are added, invert the cuvette five times to mix.
Place the cuvette into the meter. Close the lid, and press the button. Once “Add C.2”
appears, you are ready to proceed. Next we’ll add our sample. Here, we’ll
use the 0.1mL mechanical pipette to dose exactly 0.1mL of sample to our cuvette.
To use the new Hanna Instruments Micropipette, attach the pipette tip. Push the button to
the first stop, and then immerse the tip into the sample. Slowly release the button to its
original position. Then, wait two seconds to catch any drops before dispensing the sample
into the cuvette. For best results, dispense liquid until you
reach the first stop. Once the liquid is dispensed, push the button to the second stop to remove
any remaining liquid. Once the sample is added, add one packet of
reagent B to the sample. After that, cap the cuvette and shake vigorously for 15 seconds.
Wait 15 seconds for bubbles to dissipate. Place the cuvette back into the meter. Close
the lid and press the button. The meter will display the concentration of calcium in ppm.
Once the measurement is finished, dispose of your sample in accordance with local regulations.
If the sample remains in the cuvette too long, the glass may become stained permanently.
The next sample may be taken; otherwise, the meter will power down automatically after
ten minutes.
We hope you have enjoyed this instructional video. For more information, contact us, or
visit hannainst.com