hi ashley here and today i'm going to show you how to make agar you will need these things okay
let's get started first you will need to measure out 15 grams of agar agar
and then pour it directly into your 750 milliliter bottle then measure 15 grams of malt extract
and pour it directly in the bottle then measure 1.5 grams of nutritional yeast
and pour it in the bottle now measure out three cups of warm water and pour directly in the bottle
then mix the bottle up thoroughly so that everything is completely dissolved
then add an aluminum topper
now it's time to prepare the sterilizer
you will need to position mason jars around the bottle as a brace so the bottle does not fall over
then pour water up to the level of the agar in the bottle to prevent the agar from boiling over
in the bottle i also filled a few small mason jars with water that i will use later in this video
then set the pressure to 15 psi and sterilize for 45 minutes
after 45 minutes release the pressure and start to cool down the agar
then when you're able to barely handle it move it in front of a laminar flow hood or in a sterile
environment and start to prepare your petri dishes first you will need to sterilize everything
then cut open the petri dishes and lightly stack
them in front of the laminar flow hood avoid touching them as much as possible
then give the agar another shake
and fill the petri dishes from the bottom petri dish to the top petri dish as quickly as you can
next place the mason jar from the pressure cooker on each stack i believe this helps
reduce condensation and let them cool down more evenly now wait for your petri dishes to cool down
then cut 25 pieces of parafilm and wrap up all the petri dishes with parafilm
and store in a sterile container
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